Composite

Part:BBa_K2597000:Design

Designed by: Jinke Wang   Group: iGEM18_Nanjing_NFLS   (2018-10-09)


HOsite-TMCP (HO recognizable site, Cas9)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutation CTG to TAG


Source

The Cas9 gene is amplified from plasmid px458.

References