![](https://parts.igem.org/images/partbypart/icon_rna.png)
RNA
Part:BBa_K2591022:Design
Designed by: Luo Kevin Group: iGEM18_SUSTech_Shenzhen (2018-10-11)
A gRNA designed to Wnt3A, an essential gene to secretion of the canonical wnt protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
in our part, we design different gRNAs to knockout the gene which function the secretion of the Wnt3A protein, besides, we also design a gRNA to the Wnt3A gene as a positive control group to ensure the normal function of each gRNA.
Source
the sequence of gRNA is from an exon of the mouse Wnt3A gene.