Part:BBa_K259006:Design

GFP-Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 644
  

Design Notes

No additional design considerations. The parts were fused together following RFC10 assembly.

Source

GFP originates from jellyfish Aequeora victoria. For more details visit the [http://www.expasy.ch/uniprot/P42212 SwissProt entry].

The double terminator was designed by Reshma Shetty. For more details visis the original part page.

References added by REC-CHENNAI

Chalfie, M., & Kain, S. R. (2005). Green fluorescent protein: Properties, applications and protocols. John Wiley & Sons.

Green fluorescent protein as a signal for protein-protein interactions - https://onlinelibrary.wiley.com/doi/abs/10.1002/pro.5560061107

Kukar, T., Eckenrode, S., Gu, Y., Lian, W., Megginson, M., She, J., & Wu, D. (2002). Protein microarrays to detect protein-protein interactions using red and green fluorescent proteins. Analytical Biochemistry, 306(1), 50-54. https://doi.org/10.1006/abio.2002.5614

Park, S., & Raines, R. T. (2008). Green fluorescent protein as a signal for protein-protein interactions. Protein Science, 6(11), 2344-2349. https://doi.org/10.1002/pro.5560061107