Coding

Part:BBa_K2589000:Design

Designed by: John Bechtel   Group: iGEM18_WLC-Milwaukee   (2018-10-09)


X-short J-protein HIS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to test a smaller portion of the 2017 WLC-iGEM part BBa_K2452001 for functionality and subsequently worked to clone a shorter version of BBa_K2452001 resulting in this current iteration of the x-short J-protein with a C-Terminal HIS tag.


Source

Cloned via PCR from the pETail4 plasmid into pSB1C3.

References