Composite

Part:BBa_K2587030

Designed by: Sarah Seyffert   Group: iGEM18_Duesseldorf   (2018-10-04)


Pj23106_RBS_ddh_T

Transcription unit with the gene ddh coding for Meso-diaminopimelate D-dehydrogenase. The enzyme catalyzes the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate in the lysine biosynthesis pathway of Corynebacterium glutamicum1. We use it as a multi-step enzyme for the lysine biosynthesis in Escherichia coli, since the wild type normally needs five enzymes for the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 422
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 390
    Illegal NgoMIV site found at 615
    Illegal AgeI site found at 825
  • 1000
    COMPATIBLE WITH RFC[1000]


1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in Corynebacterium glutamicium." Journal of bacteriology 173.14 (1991): 4510-4516.:
https://jb.asm.org/content/173/14/4510.short

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