Coding

Part:BBa_K2587028:Design

Designed by: Sarah Seyffert   Group: iGEM18_Duesseldorf   (2018-10-04)


ddh


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 358
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 551
    Illegal AgeI site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed a side directed mutagenesis of BsaI and BbsI recognition sites in the coding sequence. Additional overhang from the CIDAR MoClo toolbox were added at the ends of the gene.


Source

Genomic sequence from Corynebacterium glutamicum.

References

1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in Corynebacterium glutamicium." Journal of bacteriology 173.14 (1991): 4510-4516.:
https://jb.asm.org/content/173/14/4510.short