Composite

Part:BBa_K2587026:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-10-01)


luxI_luxR_Plux_E_gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 829
    Illegal NheI site found at 852
    Illegal NheI site found at 2261
    Illegal NheI site found at 2284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 892
    Illegal BsaI site found at 1681
    Illegal BsaI site found at 1832
    Illegal BsaI.rc site found at 1523
    Illegal BsaI.rc site found at 1790
    Illegal BsaI.rc site found at 2960


Design Notes

The construct contains Type II S restriction sites and can be used for scarless and modular assembly of parts.


Source

The sequences for LuxI,LuxR,pLux and phiX174E are from GenBank and codon optimised for E. coli. The remaining sequences are from the CIDAR MoClo toolbox.

References