Composite

Part:BBa_K2587025:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-10-01)


luxR_Plux_gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 70
    Illegal BsaI site found at 859
    Illegal BsaI.rc site found at 701
    Illegal BsaI.rc site found at 968
    Illegal BsaI.rc site found at 1646


Design Notes

This part contains Type II S restriction sites and can be used for further scarless assembly.


Source

The sequences for LuxR and pLux are obtained from GenBank and codon optimised for E. coli. The remaining parts of the construct are from the CIDAR MoClo toolbox.

References