Coding

Part:BBa_K2587023:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-17)


E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6


Design Notes

The sequence is optimised for the use with the CIDAR MoClo toolbox.


Source

The sequence for the lysis gene E from PhiX174E is from GenBank and codon optimised for E. coli.

References