Coding

Part:BBa_K2587022:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-16)


rpaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 91
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3


Design Notes

The sequence contains Type II S restriction sites and is optimised to be used with the CIDAR MoClo toolbox.


Source

The sequence was obtained from GenBank and is codon optimised for E. coli.

References