Composite

Part:BBa_K2587021:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-17)


Plux_RBS_E_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI site found at 158
    Illegal BsaI.rc site found at 116


Design Notes

The construct is optimised for the use with the CIDAR MoClo toolbox.


Source

The pLux sequence and the Lysis Gene E sequence (codon optimised for E. coli) are from GenBank. The remaining parts are from the CIDAR MoClo toolbox.

References