Composite

Part:BBa_K2587020

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-17)


Plas_RBS_gfp_T

This construct contains GFP under the control of Plas, the promoter from the the quorum sensing system of Pseudomonas aeruginosa. Upon induction of this promoter from the two other components of the system, acyl homoserine lactone (AHL, synthesized by LasI) and LasR, GFP is produced.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 131
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 131
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 124
    Illegal BsaI.rc site found at 809


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