Composite
Part:BBa_K2587020
Designed by: Ylenia Longo Group: iGEM18_Duesseldorf (2018-09-17)
Plas_RBS_gfp_T
This construct contains GFP under the control of Plas, the promoter from the the quorum sensing system of Pseudomonas aeruginosa. Upon induction of this promoter from the two other components of the system, acyl homoserine lactone (AHL, synthesized by LasI) and LasR, GFP is produced.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 131
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 131
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 131
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 131
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 124
Illegal BsaI.rc site found at 809
[edit]
Categories
Parameters
None |