Composite

Part:BBa_K2587018:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-17)


Prpa_RBS_E_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93


Design Notes

The sequence is optimised for the use with the CIDAR MoClo Toolbox.


Source

The pRpa sequence as well as the lysis gene E sequence was obtained from GenBank and codon optimised for E. coli. The other parts are from the CIDAR MoClo toolbox.

References