Composite

Part:BBa_K2587017:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-17)


Prpa_RBS_gfp_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 729


Design Notes

The sequence is optiised for the use with the CIDAR MoClo toolbox. It contains Type II S restriction sites.


Source

The pRpa promoter sequence is from GenBank and codon optimised for E. coli. The remaining sequences are from the CIDAR MoClo toolbox.

References