Composite
Part:BBa_K2587016:Design
Designed by: Ylenia Longo Group: iGEM18_Duesseldorf (2018-09-16)
P_RBS_rpaR_T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 155
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 67
Design Notes
The part is optimised for the use Golden Gate Cloning using the CIDAR MoClo toolbox.
Source
The RpaR sequence was obtained from GenBank and codon optimised for E. coli. The remaining sequences were obtained from the CIDAR MoClo toolbox.