Composite

Part:BBa_K2587015:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-16)


P_RBS_lasI_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contains Type II S restriction sites and is optimised for usage with the CIDAR MoClo toolbox.


Source

The LasI sequence is from GenBank and codon optimised for E. coli. The remaining sequences are from the CIDAR MoClo Toolbox

References