Composite

Part:BBa_K2587014:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-16)


P_RBS_lasR_T


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 643
    Illegal SpeI site found at 802
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 643
    Illegal SpeI site found at 802
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 643
    Illegal SpeI site found at 802
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 643
    Illegal SpeI site found at 802
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 795


Design Notes

The construct contains Type II S restriciton sites and is optimised for the use with the CIDAR toolbox.


Source

The LasR sequence was obtained from GenBank and codon optimised for E. coli. The remaining parts are from the CIDAR MoClo Toolbox.

References