Composite

Part:BBa_K2587013:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-16)


P_RBS_luxI_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construct is optimised for the use with the CIDAR MoClo Toolbox. It contains Type II S restriction sites.


Source

The sequence for LuxI is obtained from Genbank and codon optimised for E. coli. The other parts are obtained from the CIDAR MoClo toolkit.

References