Coding

Part:BBa_K2587005:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-15)


lasR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731


Design Notes

The sequence contains Type II S restriction sites and is optimised for the use in the CIDAR toolbox.


Source

The sequence is from GenBank and codon optimised for E. coli.

References