Regulatory

Part:BBa_K2587002:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-15)


Plux


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 116


Design Notes

This sequence contains TypeII S restriction sites and is compatible with the use of the CIDAR toolbox, which is based on the Golden Gate cloning principle.


Source

This sequence was taken from GenBank and codon optimised for E. coli.

References