Regulatory

Part:BBa_K2586011:Design

Designed by: Janek Meißner   Group: iGEM18_Goettingen   (2018-09-13)


Palf4-RBS-gltT: Palf4 linked to a RBS linked to gltT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 163
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 163
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 163
    Illegal NgoMIV site found at 799
    Illegal NgoMIV site found at 1180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

yet to come


Source

(BBa_K2586000) ; (BBa_K2586008) and (BBa_K2586001) were used as templates to carry out a PCR. The PCR product was used as insert to clone this part into the required EcoRI and PstI digested pSB1C3 backbone.

References