Deleted
Sample Discontinued
Experience: None
Not Used
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RBS

Part:BBa_K2586009:Design

Designed by: Janek Meißner   Group: iGEM18_Goettingen   (2018-09-13)


RBS-gltT: Combining gltT with a RBS.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 125
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 125
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 125
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 125
    Illegal NgoMIV site found at 761
    Illegal NgoMIV site found at 1142
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Yet to come


Source

This part was made using (BBa_K2586001) and (BBa_K2586007) as templates to carry out a PCR. The PCR product was used as insert to clone this part into the required EcoRI and PstI digested pSB1C3 backbone.

References