Coding

Part:BBa_K258002:Design

Designed by: Ozkan IS   Group: iGEM09_METU-Gene   (2009-10-03)

Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 289
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Granulysin can be purified via nickel affinity chromatography.


Source

Granulysin is a member of the saposin-like protein (SAPLIP) family. The gene was synthesized at GENEART in pMA vector with standart Biobrick restriction sites

References

William A. Ernst, Sybille Thoma-Uszynski, Rachel Teitelbaum,Christine Ko,Dennis A. Hanson, Carol Clayberger, Alan M. Krensky, Matthias Leippe, Barry R. Bloom, Tomas Ganz,Robert L. Modlin.Granulysin, a T Cell Product, Kills Bacteria by Altering Membrane Permeability. The Journal of Immunology, 2000, 165: 7102–7108.