Coding

Part:BBa_K2578821:Design

Designed by: Yam Shing Fung   Group: iGEM18_Hong_Kong_JSS   (2018-09-14)


Protein coding sequence for Homo sapiens metallothionein 2 (MT2A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 325
    Illegal BamHI site found at 126
    Illegal BamHI site found at 533
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 477
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence was modified to suit the synthesis by IDT while retaining the original amino acid sequence.


Source

NCBI

References

[1]https://www.ncbi.nlm.nih.gov/gene/4502

[2] N Thirumoorthy, KT Manisenthil Kumar, A Shyam Sundar, L Panayappan, Malay Chatterjee (2007). Metallothionein: An overview. World journal of gastroenterology, ISSN 1007-9327

[3] Almaguer-Cantú V1, Morales-Ramos LH, Balderas-Rentería I. (2011) Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I. Water Sci Technol. 2011;63(8):1607-13.

[4] Dziegiel, P., Pula, B., Kobierzycki, C., Stasiolek, M., Podhorska-Okolow, M. (2016), Metallothioneins in Normal and Cancer Cells, Springer International Publishing, DOI: 10.1007/978-3-319-27472-0

[5] Zinc binds non-cooperatively to human liver metallothionein 2a at physiological pH. Jayawardena DP1, Heinemann IU2, Stillman MJ3