Composite
Part:BBa_K2577006
Designed by: Natalie Farny Group: iGEM18_WPI_Worcester (2018-10-10)
AraC regulated IAFGP
antifreeze and antimicrobial protein IAFGP regulated by AraC.
This graph shows the concentrations of purified proteins, including IAFGP, labeled as "IA" on the graph.
These are graphs of biofilm assays that included the protein IAFGP. The proteins were either mixed into the bacteria or it was used to coat the plate that the biofilm was going to be grown on.
This is a graph of the biofilm production with and without the expression of different antifreeze proteins. For this biofilm assay, an AraC promoter was used to regulate the protein expression.
The expression of these proteins was proven on the following gel:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1367
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1307
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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