Composite

Part:BBa_K2577006

Designed by: Natalie Farny   Group: iGEM18_WPI_Worcester   (2018-10-10)


AraC regulated IAFGP

antifreeze and antimicrobial protein IAFGP regulated by AraC.


T--WPI_Worcester--COncentrationofProtein.png

This graph shows the concentrations of purified proteins, including IAFGP, labeled as "IA" on the graph.

T--WPI_Worcester--CoatedPlates.png
T--WPI_Worcester--MixedPlate.png

These are graphs of biofilm assays that included the protein IAFGP. The proteins were either mixed into the bacteria or it was used to coat the plate that the biofilm was going to be grown on.

T--WPI_Worcester--Bioflim_Assay_Plate.png

This is a graph of the biofilm production with and without the expression of different antifreeze proteins. For this biofilm assay, an AraC promoter was used to regulate the protein expression.

The expression of these proteins was proven on the following gel:

T--WPI_Worcester--ProteinGel1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1307
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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