Coding

Part:BBa_K2572017

Designed by: Shan Jiang   Group: iGEM18_RDFZ-China   (2018-10-07)


Terminator.png

GBSV1 Nuclease

  • GBSV1 nuclease is a non-specific nuclease from the thermophilic bacteriophage GBSV1. The characterization of the recombinant GBSV1-NSN, the nuclease-encoding gene(Song et al.,2008)showed that its optimal temperature and pH were 60°C and 7.5, respectively. However, this part has not been characterized by us yet.


Secondary Structure

File:Mfold-K2572017-1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Song Q, Zhang X. Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1. BMC Biotechnology. 2008;8:43. doi:10.1186/1472-6750-8-43.

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