Coding

Part:BBa_K2570022:Design

Designed by: Luying He   Group: iGEM18_FJNU-China   (2018-10-05)


araC＋mazf+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2244
    Illegal SapI site found at 961


Design Notes

Gibson assembly


Source

Hazan R, Engelberg-Kulka H. mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria. Journal of Cell Science 118:4327-4332

Hazan R, Sat B, Engelberg-Kulka H. Escherichia coli mazEF-Mediated Cell Death Is Triggered by Various Stressful Conditions. Journal of Bacterology 186(11):3663-3669.

Schuster CF, Park JH, Prax M.Characterization of a mazEF Toxin-Antitoxin Homologue from Staphylococcus equorum. Journal of Bacteriology 195:115-125

Lewis D, Le P, Zurla C, Finzi L, Adhya S.Multilevel autoregulation of λ repressor protein CI by DNA looping in vitro. PNAS 108:14807-14812.

Wedler H, Wambutt R. A temperature-sensitive lambda cI repressor functions on a modified operator in yeast cells by masking the TATA element. NCBI 248:499-505.

References