Composite

Part:BBa_K2570011:Design

Designed by: Xueyi Yang   Group: iGEM18_FJNU-China   (2018-09-30)


dsrA+lacI+PlacI+hns+ProU+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1587
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3324
    Illegal SapI.rc site found at 154
    Illegal SapI.rc site found at 1973


Design Notes

We added restriction enzyme cutting site to the circuits and finished point mutation of hns to up to the standard of RFC10.

Source

E.coli BW25113.

References

[1] Temperature Sensing by the dsrA Promoter. JOURNAL OF BACTERIOLOGY, Nov. 2003, p. 6609–6614 DOI: 10.1128/JB.185.22.6609–6614.2003

[2] RNase III initiates rapid degradation of proU mRNA upon hypo-osmotic stress in Escherichia coli. RNA Biology