Coding

Part:BBa_K2570000:Design

Designed by: Luying He   Group: iGEM18_FJNU-China   (2018-08-14)


PAR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 247
    Illegal NotI site found at 610
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ordered the part DNA from a synthesis company and had codon optimization of the sequence.

Source

The Par (BAG13450.2) the phenyla-cetaldehyde reductase from the rose (Rosa sp.)

https://www.ncbi.nlm.nih.gov/protein/BAG13450.2?report=genbank&log$=protalign&blast_rank=1&RID=V0NV9P35014

References

1. Chen X M, Kobayashi H, Sakai M, et al. Functional characterization of rose phenylacetaldehyde reductase (PAR), an enzyme involved in the biosynthesis of the scent compound 2-phenylethanol[J]. Journal of Plant Physiology, 2011, 168(2):88-95.

2. Wang, P., Yang, X., Lin, B., Huang, J., & Tao, Y. (2017). Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metabolic Engineering, 44(August), 143–149.