Composite

Part:BBa_K2569032:Design

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-10-06)

PampC -mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 631
    Illegal AgeI site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mRFP behind constitutive promoter PdnaA, who is a strong member in our promoter family, leading to enhance expression of mRFP.

Source

The constitutive promoter PdnaA is a member of our promoter library.
And mRFP is amplified via PCR from BBa_J04450 on plate kits offered by iGEM.

References