Coding

Part:BBa_K2560269:Design

Designed by: Memduha Muratoglu   Group: iGEM18_Marburg   (2018-10-02)


tfoX (V. natriegens)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 562


Design Notes

The part contains the coding region from tfox of V. natriegens without the start codon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011). It was built with the Marburg Toolbox, a golden gate based toolbox for modular cloning. According to the Marburg Toolbox, the part is designed as a 4-part (CDS).

Iif there is no need for tagging, use this part.

GGTCTCGAATG-coding_region-GCTTTGAGACC


Source

genomic DNA of ATCC 14048

References