Other
Part:BBa_K2560127:Design
Designed by: Tobias Hensel Group: iGEM18_Marburg (2018-09-22)
Phytobrick version of Carbenicillin Resistance in BBa_K2560002
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created by polymerase chain reaction (PCR) on the template pTrc-McrCa and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.
Forward oligo: TTCGTCTCCCTCATGTTTTCAAATATGTATCCGCTCATGAGAC
Reverse Oligo: TTCGTCTCCCGTGACCCACGCTCACC
Source
As Source DNA we used pTrc-McrCa from the Erb Lab in Marburg, Germany.