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Part:BBa_K2560105:Design
Designed by: Tobias Hensel Group: iGEM18_Marburg (2018-09-22)
Phytobrick version of 5'Con5'_L1_inv_Ori Connector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our Design Page.
Source
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.
Forward Oligo: CTCGAACACGTCTCGAGCTGGAG
Reverse Oligo: CTCACTCCAGCTCGAGACGTGTT