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Part:BBa_K2560105:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-09-22)


Phytobrick version of 5'Con5'_L1_inv_Ori Connector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our Design Page.

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGAACACGTCTCGAGCTGGAG

Reverse Oligo: CTCACTCCAGCTCGAGACGTGTT