Part:BBa_K2560094:Design

Phytobrick version of 5b BBa_B1006

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence was obtained from part BBa_B1006. The parts sequence was not changed apart from adding the terminator overhangs that are required for subsequent cloning.

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGGGTAAAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTTCGCT

Reverse Oligo: CTCAAGCGAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTTTACC