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Part:BBa_K2560051:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-09-18)


Phytobrick version of Lux Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2928
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3447


Design Notes

This part was created by polymerase chain reaction out of two Parts on the template PsV0-15-024 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Part 1:

Forward oligo: AACGTCTCGCTCGAATGACTAAAAAAATTTCATTCATTATTAACGGCCAGG

Reverse Oligo: AACGTCTCGACGAGTCTCTTGTTTAGCTTTATTACTATCTTCG


Part 2:

Forward oligo: AACGTCTCGTCGTGCATTTATTAGTGATTATGTTCTTGAAATGC

Reverse Oligo: TTCGTCTCCCTCAAAGCACTATCAAACGCTTCGGTTAAGCTC

Source

As source DNA we used PsV0-15-024 from the Fritz Lab in Marburg, Germany.