Coding

Part:BBa_K2560047:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-09-18)


Phytobrick version of Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A Cas9 and dCas9 LVL0 part was created as a CDS part in our Marburg Collection. The cas9 sequence was PCR amplified from pCas9CR4 (Reisch and Prather 2017). To make this sequence compatible with our toolbox and the iGEM registry, we removed a BsmBI and EcoRI recognition site in the parts sequence creating the Cas9 LVL0 part BBa_K2560047.