Coding
Part:BBa_K2560043:Design
Designed by: Tobias Hensel Group: iGEM18_Marburg (2018-10-09)
Phytobrick version of sfGFP Vn
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created by polymerase chain reaction (PCR) on the template BBa_K2560305 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.
Forward oligo: AACGTCTCGCTCGAATGCGTAAAGGCGAAGAACTTTTC
Reverse Oligo: TTCGTCTCCCTCAAAGCTTTATACAGCTCATCCATGCCG
Source
The Source DNA were bought from IDT.