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Part:BBa_K2560012:Design
Designed by: Tobias Hensel Group: iGEM18_Marburg (2018-08-21)
Phytobrick version of 3'Connector Dummy
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 2
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our Design Page.
Source
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.
Forward Oligo: CTCGCGCTTACTAGTAGCGGCCGCTGCAGAGCT
Reverse Oligo: CTCAAGCTCTGCAGCGGCCGCTACTAGTAAGCG