Composite

Part:BBa_K2558216:Design

Designed by: Tsinghua 2018   Group: iGEM18_Tsinghua   (2018-10-05)

CRISPRi Safety Catch device with lux pR-HS promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 310
    Illegal NheI site found at 333
    Illegal NheI site found at 1466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5850
    Illegal BamHI site found at 3745
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 79
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4617


Design Note

Assisted with the experiences we gained form the experiments above, we built and tuned the NEON system. We designed this experiment to characterize how Neon the positive feedback plasmid (BBa_K2558214), and Safety Catch the CRISPRi plasmid (BBa_K2558215, BBa_K2558216) work together.

Source

References