DNA

Part:BBa_K2557041:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


Bxb1 attB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36


Design Notes

To facilitate cloning, we randomly added some bases on both sides of attB.


Source

After the recombination site of Bxb1 was found in the literature, it was sent to Kingsray for synthesis.

References