Composite

Part:BBa_K2557029:Design

Designed by: Wang Chunfeng   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-EF1α promoter -EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 499
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 505
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to facilitate the construction of TetO-EF1αpromoter -recombinase parts, We added the TetO sequence before the EF1α promoter. However, in the absence of TetR, The TetO sequence does not affect the strength of the promoter, that is, it does not affect the expression of EGFP.


Source

The EF1α promoter and EFGP were cloned from a plasmid borrowed from other laboratories in the school.

References