Composite

Part:BBa_K2557028:Design

Designed by: Wang Chunfeng   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-miniCMV promoter- EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 331
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to facilitate the construction of TetO-miniCMV promoter -recombinase parts, We added the TetO sequence before the miniCMV promoter. However, in the absence of TetR, The TetO sequence does not affect the strength of the promoter, that is, it does not affect the expression of EGFP.


Source

The miniCMV promoter and EFGP were cloned from a plasmid borrowed from other laboratories in the school.

References