Composite

Part:BBa_K2557019:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


miniCMV promoter-Bxb1-RDF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1612
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1612
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 820
    Illegal BglII site found at 1618
    Illegal BamHI site found at 46
    Illegal BamHI site found at 261
    Illegal BamHI site found at 577
    Illegal BamHI site found at 1285
    Illegal BamHI site found at 1576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1612
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 2095
    Illegal NgoMIV site found at 2278
    Illegal AgeI site found at 52
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 282


Design Notes

In order to allow the recombinase to enter the nucleus and function, we added SV40 NLS to the front end. To detect protein content, we added the FLAG tag.


Source

The miniCMV promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the sequence of Bxb1-RDF from the literature and synthesized this sequence.

References