Composite

Part:BBa_K2557018:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-Ubc promoter-TP901


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 753
    Illegal PstI site found at 1326
    Illegal PstI site found at 2037
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1326
    Illegal PstI site found at 2037
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1348
    Illegal BamHI site found at 687
    Illegal BamHI site found at 2292
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 753
    Illegal PstI site found at 1326
    Illegal PstI site found at 2037
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 753
    Illegal PstI site found at 1326
    Illegal PstI site found at 2037
    Illegal NgoMIV site found at 792
    Illegal NgoMIV site found at 936
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1545


Design Notes

SV40 NLS and flag was added by PCR.


Source

The TetO sequence was cloned from a Commercialized plasmid. The Ubc promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of TP901on NCBI and, after humanization, sent it to Kingsray for synthesis.

References