Composite

Part:BBa_K2557015:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-Ubc promoter-PhiC31


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 754
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 687
    Illegal BamHI site found at 1605
    Illegal BamHI site found at 1691
    Illegal BamHI site found at 1938
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 754
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 754
    Illegal NgoMIV site found at 772
    Illegal NgoMIV site found at 1001
    Illegal NgoMIV site found at 1043
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1297
    Illegal BsaI.rc site found at 1700
    Illegal BsaI.rc site found at 2053


Design Notes

SV40 NLS and flag was added by PCR.


Source

The TetO sequence was cloned from a Commercialized plasmid. The Ubc promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of PhiC31 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References