Composite

Part:BBa_K2557012:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-Ubc promoter-Bxb1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 753
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
    Illegal BamHI site found at 687
    Illegal BamHI site found at 908
    Illegal BamHI site found at 1224
    Illegal BamHI site found at 1932
    Illegal BamHI site found at 2223
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 753
    Illegal NgoMIV site found at 1209
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 929


Design Notes

SV40 NLS and flag was added by PCR.


Source

The TetO sequence was cloned from a Commercialized plasmid. The Ubc promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of Bxb1 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References