Composite
Part:BBa_K2557010:Design
Designed by: Ge JT Group: iGEM18_NAU-CHINA (2018-10-09)
TetO-miniCMV promoter-Bxb1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1099
Illegal BamHI site found at 325
Illegal BamHI site found at 540
Illegal BamHI site found at 856
Illegal BamHI site found at 1564
Illegal BamHI site found at 1855 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 841
Illegal AgeI site found at 331 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 561
Design Notes
SV40 NLS and flag was added by PCR.
Source
The TetO sequence was cloned from a Commercialized plasmid. The miniCMV promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of Bxb1 on NCBI and, after humanization, sent it to Kingsray for synthesis.