DNA

Part:BBa_K2557006:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


PhiC31 attB-PhiC31 attP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 92
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To facilitate cloning, we randomly added some bases on both sides of attB and attP.


Source

After the recombination site of PhiC31 was found in the literature, it was sent to Kingsray for synthesis.

References