Part:BBa_K2557002:Design

Bxb1 attB-RFP-Bxb1 attP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 837
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 36
  Illegal BsaI site found at 177
  Illegal BsaI.rc site found at 885
  Illegal SapI site found at 794
  

Design Notes

The sequence of the RFP is inverted. To facilitate cloning, we randomly added some bases on both sides of attB and attP.

Source

After the recombination site of Bxb1 was found in the literature, it was sent to Kingsray for synthesis.