Coding
Part:BBa_K2557000:Design
Designed by: Ge JT Group: iGEM18_NAU-CHINA (2018-10-04)
Anti-GFP-mnotch-TEV protease-NLS
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 409
Illegal PstI site found at 453
Illegal PstI site found at 772 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 16
Illegal PstI site found at 409
Illegal PstI site found at 453
Illegal PstI site found at 772
Illegal NotI site found at 1570 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2063
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 409
Illegal PstI site found at 453
Illegal PstI site found at 772 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 409
Illegal PstI site found at 453
Illegal PstI site found at 772
Illegal NgoMIV site found at 208
Illegal NgoMIV site found at 913
Illegal NgoMIV site found at 1090
Illegal NgoMIV site found at 1628
Illegal AgeI site found at 25 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1249
Design Notes
Since we need TEV protease to enter the nucleus, we added SV40 NLS to the back of the TEV protease.
Source
We borrowed the Anti-GFP-mnotch-tetR-VP64-V2 plasmid from Fudan University. After the TEV protease sequence used by the iGEM17_Oxford group was found on the iGEM website, it was sent to Kingsray for synthesis.